NTP compares chemical and biological profiles of botanical supplements
National Toxicology Program (NTP) researchers used chemical and biological response profiles to evaluate the similarity of two botanical supplements with well-established NTP test articles. The approaches they used could be applied to other types of complex mixtures with unidentified active components. The scientists suggested that confirming similarity was an important step in selecting test articles and extrapolating from a tested mixture to related samples.
Researchers chose two botanical supplements, black cohosh (Actaea racemosa) and Echinacea purpurea, to analyze variability between samples. The scientists used nontargeted chemical analysis to create chemical profiles and gene expression of toxicologically important receptor pathways in human liver cells to assess bioactivity.
The combined chemical and biological data for black cohosh identified nine of 14 samples that were similar to the control. Five of 14 samples were similar in terms of both chemistry and bioactivity for Echinacea purpurea. Together, these results indicated that the approaches for determining sufficient similarity were useful for evaluating both black cohosh and Echinacea purpurea. The authors asserted that multiple techniques must be used to confirm sample similarity. (KH)
Citation: Ryan KR, Huang MC, Ferguson SS, Waidyanatha S, Ramaiahgari S, Rice JR, Dunlap PE, Auerbach SS, Mutlu E, Cristy T, Peirfelice J, DeVito MJ, Smith-Roe SL, Rider CV. 2019. Evaluating sufficient similarity of botanical dietary supplements: combining chemical and in vitro biological data. Toxicol Sci; doi: 10.1093/toxsci/kfz189 [Online 27 August 2019].