NIEHS scientists were treated to a day-long showcase featuring in-house resources for the increasingly vital study of genomes and epigenomes. Epigenetics refers to changes to DNA that do not affect the underlying genes, but do affect their activity. Anchored by a keynote presentation from NIEHS Acting Deputy Scientific Director Paul Wade, Ph.D., the May 11 event offered a wealth of scientific findings and technology updates.
Complementing the presentations were nearly 70 posters displayed by researchers and trainees in the NIEHS Division of Intramural Research and Clinical Research Branch, the National Toxicology Program, and vendors.
Cores offer cutting-edge technology and services
Lead organizer Kevin Gerrish, Ph.D., acting director of the NIEHS Molecular Genomics Core Laboratory, kicked off the day with a welcome and thanks for the staff of the core. "They do the real work," he said.
Greg Solomon, manager of the Epigenomics and DNA Sequencing Core Laboratory, and Jason Li, Ph.D., director of the Integrative Bioinformatics Support Group, described the analyses and support their groups provide, recent growth in operations, and how to best use their resources.
The Epigenomics Core offers several high-throughput DNA sequencing technologies, and Solomon noted the increase of requests in recent years (see graph, right). This is undoubtedly due to the changing nature of biomedical research, but his service-oriented attitude is at least partially responsible. "If there’s a technique you’d like to do, let us know, and we’ll learn how to do it," he told the audience.
Li, who stepped into his director role in 2016, explained that his group provides support to researchers as needed, and for long-term projects, includes informaticians within the research groups. The team consults on research design, analytical strategies, data analysis, training, and outreach. Scientists in the core also develop software and tools needed by environmental health scientists, such as the recently released Online Resource for Integrative Omics (ORIO) software package (see summary).
ORIO, published in April, was discussed in more detail by Christopher "Andy" Lavender, Ph.D., from Li’s group. Other talks (see text box) by trainees and researchers, despite their wide-ranging topics and techniques, all featured research that relied on the services of NIEHS core laboratories.
DNA methylation in parents and offspring
Patterns of DNA methylation, which is one form of epigenetic change, can provide information about the individual being studied, as well as their parents, Wade explained in his talk. Ongoing research in his lab, the Eukaryotic Transcriptional Regulation Group, examines how methylation patterns in parents influence those of offspring and whether those patterns provide a record of environmental exposures, such as endocrine disrupting chemicals and obesity.
"DNA methylation has rules about how it is inherited from parent to progeny, it is stable under controlled circumstances, and it does provide a long-lasting record of major life events, including obesity and diet," Wade concluded, thanking by name the individuals who helped design the experiments and analyze the data, with much of the sequencing done in-house at NIEHS.